abstract：:Five nominally freshwater microalgae (Chlorella vulgaris, Choricystis minor, Neochloris sp., Pseudococcomyxa simplex, Scenedesmus sp.) with a known ability to produce high-levels of lipids for possible use as fuel oils were evaluated for their ability to thrive and produce lipids in seawater and brackish water. Only C...

journal_title：Journal of biotechnology

authors： Luangpipat T,Chisti Y

更新日期：2017-09-10 00:00:00

abstract：:Extracellular enzymes are assumed to be responsible for the initial and rate limiting step in biodegradation of polymers. Mainly enzymes with aliphatic esters as their natural substrates (e.g. lipase, cutinases) have until now been evaluated for polyester hydrolysis studies. However, the potential of enzymes with arom...

journal_title：Journal of biotechnology

authors： Haernvall K,Zitzenbacher S,Yamamoto M,Schick MB,Ribitsch D,Guebitz GM

更新日期：2017-09-10 00:00:00

abstract：:The polysaccharide xanthan which is produced by the γ-proteobacterium Xanthomonas campestris is used as a food thickening agent and rheologic modifier in numerous food, cosmetics and technical applications. Its great commercial importance stimulated biotechnological approaches to optimize the xanthan production. By ta...

journal_title：Journal of biotechnology

authors： Teckentrup J,Al-Hammood O,Steffens T,Bednarz H,Walhorn V,Niehaus K,Anselmetti D

更新日期：2017-09-10 00:00:00

abstract：:The first complete genome sequence of Bacillus glycinifermentans B-27 was determined by SMRT sequencing generating a genome sequence with a total length of 4,607,442 bases. Based on this sequence 4738 protein-coding sequences were predicted and used to identify gene clusters that are related to the production of secon...

journal_title：Journal of biotechnology

authors： Stadermann KB,Blom J,Borgmeier C,Sciberras N,Herbold S,Kipker M,Meurer G,Molck S,Petri D,Pelzer S,Schneider J

更新日期：2017-09-10 00:00:00

abstract：:Herbinix hemicellulosilytica is a newly isolated, gram-positive, anaerobic bacterium with extensive hemicellulose-degrading capabilities obtained from a thermophilic biogas reactor. In order to exploit its potential as a source for new industrial arabinoxylan-degrading enzymes, six new thermophilic xylanases, four fro...

journal_title：Journal of biotechnology

authors： Mechelke M,Koeck DE,Broeker J,Roessler B,Krabichler F,Schwarz WH,Zverlov VV,Liebl W

更新日期：2017-09-10 00:00:00

abstract：:We introduce an easy, fast and effective method to analyze the influence of genetically modified (GM) plants on soil and model organisms in the laboratory to substitute laborious and time consuming field trials. For the studies described here we focused on two GM plants of the so-called 3rd generation: GM plants produ...

journal_title：Journal of biotechnology

authors： Horn P,Schlichting A,Baum C,Hammesfahr U,Thiele-Bruhn S,Leinweber P,Broer I

更新日期：2017-09-10 00:00:00

abstract：:Anaerobic hydrocarbon biodegradation not only diminishes fuel quality, but also exacerbates the biocorrosion of the metallic infrastructure. While successional events in marine microbial ecosystems impacted by petroleum are well documented, far less is known about the response of communities chronically exposed to hyd...

journal_title：Journal of biotechnology

authors： Liang R,Duncan KE,Le Borgne S,Davidova I,Yakimov MM,Suflita JM

更新日期：2017-08-20 00:00:00

abstract：:Deployment of the elongated flexuous virions of Turnip mosaic virus (TuMV), a potyvirus, for peptide display on their external surface has been previously reported by us. Nonetheless, both in TuMV and other potyviruses some peptides hinder the ability of the virus to infect host plants. We found that a peptide derived...

journal_title：Journal of biotechnology

authors： González-Gamboa I,Manrique P,Sánchez F,Ponz F

更新日期：2017-07-20 00:00:00

abstract：:Aspergillus oryzae finds wide application in the food, feed, and wine industries, and is an excellent cell factory platform for production of organic acids. In this work, we achieved the overproduction of L-malate by rewiring the reductive tricarboxylic acid (rTCA) pathway and L-malate transport pathway of A. oryzae N...

journal_title：Journal of biotechnology

authors： Liu J,Xie Z,Shin HD,Li J,Du G,Chen J,Liu L

更新日期：2017-07-10 00:00:00

abstract：:This study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (E)-11-(heptanoyloxy) undec-9-enoic acid (11-HOUA), a key intermediate of brassylic acid, by recombinant Escherichia coli overexpressing an alcohol dehydrogenase from Micrococcus l...

journal_title：Journal of biotechnology

authors： Cho YH,Kim SJ,Kim HW,Kim JY,Gwak JS,Chung D,Kim KH,Park K,Park YC

更新日期：2017-07-10 00:00:00

abstract：:Recombinant antibody fragments belong to the promising class of biopharmaceuticals with high potential for future therapeutic applications. However, due to their small size they are rapidly cleared from circulation. Binding to serum proteins can be an effective approach to improve pharmacokinetic properties of short h...

journal_title：Journal of biotechnology

authors： Cantante C,Lourenço S,Morais M,Leandro J,Gano L,Silva N,Leandro P,Serrano M,Henriques AO,Andre A,Cunha-Santos C,Fontes C,Correia JDG,Aires-da-Silva F,Goncalves J

更新日期：2017-07-10 00:00:00

abstract：:The styrene oxide isomerase (SOI, StyC) represents a key enzyme of the styrene-degrading pathway and has been discussed as promising biocatalyst during recent studies. The enzyme enables the synthesis of pure phenylacetaldehyde from styrene oxide. In this study the native as well as the corresponding codon-optimized g...

journal_title：Journal of biotechnology

authors： Oelschlägel M,Richter L,Stuhr A,Hofmann S,Schlömann M

更新日期：2017-06-20 00:00:00

abstract：:Actinoplanes sp. SE50/110 is the natural producer of acarbose, which is used in the treatment of diabetes mellitus type II. However, until now the transcriptional organization and regulation of the acarbose biosynthesis are only understood rudimentarily. The genome sequence of Actinoplanes sp. SE50/110 was known befor...

journal_title：Journal of biotechnology

authors： Wolf T,Schneiker-Bekel S,Neshat A,Ortseifen V,Wibberg D,Zemke T,Pühler A,Kalinowski J

更新日期：2017-06-10 00:00:00

abstract：:Understanding the causes of discrepancies in pH readings of a sample can allow more robust pH control strategies to be implemented. It was found that 59.4% of differences between two offline pH measurement technologies for an historical dataset lay outside an expected instrument error range of ±0.02pH. A new variable,...

journal_title：Journal of biotechnology

authors： Ritchie EK,Martin EB,Racher A,Jaques C

更新日期：2017-06-10 00:00:00

abstract：:Fast turnaround times are of utmost importance for biomedical reconnaissance, particularly regarding dangerous pathogens. Recent advances in sequencing technology and its devices allow sequencing within a short time frame outside stationary laboratories close to the epicenter of the outbreak. In our study, we evaluate...

journal_title：Journal of biotechnology

authors： Walter MC,Zwirglmaier K,Vette P,Holowachuk SA,Stoecker K,Genzel GH,Antwerpen MH

更新日期：2017-05-20 00:00:00

abstract：:Whole metagenome shotgun sequencing provides information about the gene content and the composition of microbial communities provided that the processing of the samples does not introduce a methodology-driven bias. We tested the impact of DNA isolation and storage period on the metagenome profile. Deep throat swabs we...

journal_title：Journal of biotechnology

authors： Wiehlmann L,Pienkowska K,Hedtfeld S,Dorda M,Tümmler B

更新日期：2017-05-20 00:00:00

abstract：:One important question in microbiome analysis is how to assess the homogeneity of the microbial composition in a given environment, with respect to a given analysis method. Do different microbial samples taken from the same environment follow the same taxonomic distribution of organisms, or the same distribution of fu...

journal_title：Journal of biotechnology

authors： Huson DH,Steel M,El-Hadidi M,Mitra S,Peter S,Willmann M

更新日期：2017-05-20 00:00:00

abstract：:Protein modifications by intricate cellular machineries often redesign the structure and function of existing proteins to impact biological networks. Disulfide bond formation between cysteine (Cys) pairs is one of the most common modifications found in extracellularly-destined proteins, key to maintaining protein stru...

journal_title：Journal of biotechnology

authors： Zhong X,He T,Prashad AS,Wang W,Cohen J,Ferguson D,Tam AS,Sousa E,Lin L,Tchistiakova L,Gatto S,D'Antona A,Luan YT,Ma W,Zollner R,Zhou J,Arve B,Somers W,Kriz R

更新日期：2017-04-20 00:00:00

abstract：:Glycosylation is a prominent biological mechanism for structural and functional diversity of natural products. Uridine diphosphate-dependent glycosyltransferases with aglycon promiscuity are generally recognised as effective biocatalysts for glycodiversification of natural products for practical applications. In this ...

journal_title：Journal of biotechnology

authors： Dai L,Li J,Yao P,Zhu Y,Men Y,Zeng Y,Yang J,Sun Y

更新日期：2017-04-20 00:00:00

abstract：:Microbiologically induced calcium carbonate precipitation shows the potential for use in bioremediation and construction consolidation, but the efficiency of this process must be improved. Lysinibacillus sphaericus LMG 22257 is a gram-positive ureolytic strain that has recently been applied for consolidating construct...

journal_title：Journal of biotechnology

authors： Yan W,Xiao X,Zhang Y

更新日期：2017-03-20 00:00:00

abstract：:Consumers prefer biotechnological food products with high nutritional values and good flavors. Solid-state fermentation is a commonly used technique with a long history. In the present study, Myroides sp. ZB35 was used in solid-state fermentative production of aroma volatiles on a rice medium. Using the headspace soli...

journal_title：Journal of biotechnology

authors： Xiao Z,Liang W,Zhu X,Zhao JY

更新日期：2017-03-10 00:00:00

abstract：:Enantiomers of 3-hydroxybutyric acid (3-HB) can be used as the chiral precursors for the production of various optically active fine chemicals, including drugs, perfumes, and pheromones. In this study, Escherichia coli was engineered to produce (S)-3-HB from glucose through the inverted reactions of the native aerobic...

journal_title：Journal of biotechnology

authors： Gulevich AY,Skorokhodova AY,Sukhozhenko AV,Debabov VG

更新日期：2017-02-20 00:00:00

abstract：:Optically pure 1-phenyl-1,2-ethanediol is a very important chiral building block and intermediate in fine chemical and pharmaceutical industries. Reduction of 2-hydroxyacetophenone provides a straightforward approach to access these important compounds. In this study, two enantiocomplementary carbonyl reductases, BDHA...

journal_title：Journal of biotechnology

authors： Cui ZM,Zhang JD,Fan XJ,Zheng GW,Chang HH,Wei WL

更新日期：2017-02-10 00:00:00

abstract：:Fast and reliable methods to determine biomass concentration are necessary to facilitate the large scale production of microalgae. A method for the rapid estimation of Chlorella sorokiniana biomass concentration was developed. The method translates the suspension particle size spectrum gathered though laser reflectanc...

journal_title：Journal of biotechnology

authors： López Expósito P,Blanco Suárez A,Negro Álvarez C

更新日期：2017-02-10 00:00:00

abstract：:In this study the ability of CYP109E1 from Bacillus megaterium to metabolize vitamin D3 (VD3) was investigated. In an in vitro system using bovine adrenodoxin reductase (AdR) and adrenodoxin (Adx4-108), VD3 was converted by CYP109E1 into several products. Furthermore, a whole-cell system in B. megaterium MS941 was est...

journal_title：Journal of biotechnology

authors： Abdulmughni A,Jóźwik IK,Putkaradze N,Brill E,Zapp J,Thunnissen AW,Hannemann F,Bernhardt R

更新日期：2017-02-10 00:00:00

abstract：:The marine microalga Nannochloropsis oculata is a potential source of eicosapentaenoic acid (EPA, C20:5n3) and carotenoids for use in functional foods and nutraceuticals. Mixotrophic culture of N. oculata using glycerol was examined as a possible way of increasing the biomass and metabolite productivity relative to a ...

journal_title：Journal of biotechnology

authors： Shene C,Chisti Y,Vergara D,Burgos-Díaz C,Rubilar M,Bustamante M

更新日期：2016-12-10 00:00:00

abstract：:The genus Ornithogalum includes several ornamental species that suffer substantial losses from bacterial soft rot caused by Pectobacteria. The absence of effective control measures for use against soft rot bacteria led to the initiation of a project in which a small antimicrobial peptide from an Asian horseshoe crab, ...

journal_title：Journal of biotechnology

authors： Lipsky A,Joshi JR,Carmi N,Yedidia I

更新日期：2016-11-20 00:00:00

abstract：:In this study we focused on identifying and characterizing polydimethylsiloxane-binding peptides (PDMS-tags) that show a strong binding affinity towards a PDMS surface. Three kinds of E. coli host proteins (ELN, OMC and TPA) that were preferentially adsorbed onto a PDMS surface were identified from the E. coli cell ly...

journal_title：Journal of biotechnology

authors： Kumada Y,Otsuki R,Sakoda Y,Akai R,Matoba K,Katayama J,Kishimoto M,Horiuchi J

更新日期：2016-10-20 00:00:00

abstract：:Oil sands tailings ponds store the waste slurry generated by extracting bitumen from surface-mined oil (tar) sands ores. The ponds support diverse microbial communities involved in element cycling, greenhouse gas production, and hydrocarbon biodegradation that influence pond management and their environmental footprin...

journal_title：Journal of biotechnology

authors： Wilson SL,Li C,Ramos-Padrón E,Nesbø C,Soh J,Sensen CW,Voordouw G,Foght J,Gieg LM

更新日期：2016-10-10 00:00:00

abstract：:Hydroxynitrile lyases (HNLs) catalyze the asymmetric addition of HCN to aldehydes producing enantiomerically pure cyanohydrins. These enzymes can be heterologously expressed in large quantities making them interesting candidates for industrial applications. The HNLs from Rosaceae evolved from flavin dependent dehydrog...

journal_title：Journal of biotechnology

authors： Pavkov-Keller T,Bakhuis J,Steinkellner G,Jolink F,Keijmel E,Birner-Gruenberger R,Gruber K

更新日期：2016-10-10 00:00:00

abstract：:The production of isoprenoids in recombinant microbes for flavor & fragrance, pharmaceutical, agricultural or fuel applications is a booming research field. Isoprenoid extraction from natural resources and chemical synthesis is frequently neither ecological nor commercially profitable. However, recombinant microbes al...

journal_title：Journal of biotechnology

authors： Emmerstorfer-Augustin A,Moser S,Pichler H

更新日期：2016-10-10 00:00:00

abstract：:New antifungal compounds that circumvent the resistance of the pathogen by directly damaging yeast cell surface structures are promising agents for the treatment of fungal infections, due to their different mechanism of action from current clinically used antifungal drugs. We present here a rapid and cost-effective fl...

journal_title：Journal of biotechnology

authors： Kodedová M,Sychrová H

更新日期：2016-09-10 00:00:00

abstract：:To study the metaproteome of a biogas-producing microbial community, fermentation samples were taken from an agricultural biogas plant for microbial cell and protein extraction and corresponding metagenome analyses. Based on metagenome sequence data, taxonomic community profiling was performed to elucidate the composi...

journal_title：Journal of biotechnology

authors： Ortseifen V,Stolze Y,Maus I,Sczyrba A,Bremges A,Albaum SP,Jaenicke S,Fracowiak J,Pühler A,Schlüter A

更新日期：2016-08-10 00:00:00

abstract：:Acinetobacter baumannii has turned into an important threat in nosocomial outbreak infections and multidrug resistance leading to high mortality rates in the 21st century. In recent years its mortality has increased by 15% which in part could be due to lack of a rapid and sensitive diagnostic test. In this work we int...

journal_title：Journal of biotechnology

authors： Rasoulinejad S,Gargari SLM

更新日期：2016-08-10 00:00:00

abstract：:Vertebrate prostaglandin H synthases (PGHSs) are membrane-bound disulphide-containing hemoglycoproteins. Therefore, eukaryotic expression systems are required for the production of recombinant PGHSs. Recently we announced the expression of human PGHS-2 (hPGHS-2) in the yeast Pichia pastoris. Here we report improved pr...

journal_title：Journal of biotechnology

authors： Kukk K,Samel N

更新日期：2016-08-10 00:00:00

abstract：:Saccharum spontaneum L., a wild relative of sugarcane, is known for its adaptability to environmental stresses, particularly cold stress. In the present study, an attempt was made for transcriptome profiling of the low temperature (10°C) tolerant S. spontaneum clone IND 00-1037 collected from high altitude regions of ...

journal_title：Journal of biotechnology

authors： Dharshini S,Chakravarthi M,J AN,Manoj VM,Naveenarani M,Kumar R,Meena M,Ram B,Appunu C

更新日期：2016-08-10 00:00:00

abstract：:The present proof-of-concept study reports the construction of a whole-cell biocatalyst for the de novo production of ω-hydroxy octanoic acid. This was achieved by hijacking the natural fatty acid cycle and subsequent hydroxylation using a specific monooxygenase without the need for the additional feed of alkene-like ...

journal_title：Journal of biotechnology

authors： Kirtz M,Klebensberger J,Otte KB,Richter SM,Hauer B

更新日期：2016-07-20 00:00:00

abstract：:In the important industrial yeast Saccharomyces cerevisiae, galactose metabolism requires energy production by respiration; therefore, this yeast cannot metabolize galactose under strict anaerobic conditions. While the respiratory dependence of galactose metabolism provides benefits in terms of cell growth and populat...

journal_title：Journal of biotechnology

authors： Quarterman J,Skerker JM,Feng X,Liu IY,Zhao H,Arkin AP,Jin YS

更新日期：2016-07-10 00:00:00

abstract：:The action of three glycoside hydrolase family GH51 and one GH54 α-arabinofuranosidases (ABFs) was studied on polymeric arabinoxylan and arabinoxylooligosaccharides. The substrates covered all possible arabinofuranosyl (Araf) substituents, i.e., terminal and internal α(1→2) and α(1→3)-linked Araf monosubstitutions and...

journal_title：Journal of biotechnology

authors： Koutaniemi S,Tenkanen M

更新日期：2016-07-10 00:00:00

abstract：:Oleaginous microalgae have received a considerable attention as potential biofuel feedstock. However, lack of industry-suitable strain with lipid rich biomass limits its commercial applications. Targeted engineering of lipogenic pathways represents a promising strategy to enhance the efficacy of microalgal oil product...

journal_title：Journal of biotechnology

authors： Li DW,Cen SY,Liu YH,Balamurugan S,Zheng XY,Alimujiang A,Yang WD,Liu JS,Li HY

更新日期：2016-07-10 00:00:00